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Chemical Senses 23: 249-255,
© 1998 Oxford University Press

Current Status of Antisense DNA Methods in Behavioral Studies

Sonoko Ogawa and Donald W. Pfaff

Laboratory of Neurobiology and Behavior, The Rockefeller University 1230 York Avenue, New York, NY 10021, USA

Correspondence to be sent to: Sonoko Ogawa, Laboratoty of Neurobiology and Behavior; The Rockefeller University Box 275, 1230 York Avenue, New York, NY 10021, USA. e-mail: ogawa{at}rockvax.rockefeller.edu


  Abstract

The antisense DNA method has been used successfully to block the expression of specific genes in vivo in neuronal systems. An increasing number of studies in the last few years have shown that antisense DNA administered directly into the brain can modify various kinds of behaviors. These findings strongly suggest that the antisense DNA method can be used as a powerful tool to study causal relationships between molecular processes in the brain and behavior. In this article we review the current status of the antisense method in behavioral studies and discuss its potentials and problems by focusing on the following four aspects: (i) optimal application paradigms of antisense DNA methods in behavioral studies; (U) efficiencies of different administration methods of antisense DNA used in behavioral studies; (iii) determination of specificity of behavioral effects of antisense DNA; and (iv) discrepancies between antisense DNA effects on behaviors and those on protein levels of the targeted gene.

Accepted 30 July 1997


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