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Chemical Senses 23: 363-369,
© 1998 Oxford University Press

Electrophysiological Properties of Frog Olfactory Supporting Cells

Didier Trotier

Neurobiologie Sensorielle E.P.H.E., 1 avenue des Olympiades, 91300 Massy, France

Correspondence to be sent to: Didier Trotier, Laboratoire de Neurobiologie Sensorielle, Ecole Pratique des Hautes Etudes, 1 Avenue des Olympiades, F-91300 Massy, France. e-mail: trotier{at}bisance.citi2.fr


  Abstract

Cells, identified as supporting cells by Lucifer Yellow injection, were recorded from slices of frog olfactory epithelium using patch-clamp recordings. Cell-attached single-channel recordings indicated that the intracellular potential (IP) was –68 ± 7 mV (n = 22) with 4 mM K+ in the bath ([K+]0). IP was –67 ± 4 mV (n = 32) in whole-cell conditions with 100 mM KCI inside the cell, suggesting a low membrane permeability for Cl–. IP depended on [K+]0 in a manner described by the Goldman- Hodgkin-Katz equation with a permeability ratio pK+:pNa+ of 40. The input resistance was 32 ± 14 M{omega} (n = 15), indicating a high membrane conductance at rest. Odorant stimulations evoked passive membrane depolarizations, probably reflecting an increase in [K+]0 due to the neuronal activation. Whole-cell recordings with 100 mM CsCl instead of KCl in the pipette, together with the block of gap-junctions with octanol, indicated the existence of an electrical coupling between supporting cells. The electrical coupling between these glial-like cells could facilitate the clearance of K+ ions released by olfactory receptor neurons during odorant stimulation.

Accepted 24 February 1998


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