Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Harder, D. B.
Right arrow Articles by Whitney, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Harder, D. B.
Right arrow Articles by Whitney, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Chem. Senses 25: 39-45, 2000
© Oxford University Press 2000

Sucrose Octaacetate Avoidance in Nontaster Mice is not Enhanced by Two Type-A Prp Transgenes from Taster Mice

David B. Harder, Edwin A. Azen1 and Glayde Whitney

Department of Psychology, Florida State University, Tallahassee, FL 32306-1270 1 Departments of Medicine and Medical Genetics, University of Wisconsin, Madison, WI 53706, USA

Correspondence to be sent to: Edwin A. Azen, University of Wisconsin, Department of Medicine, 1300 University Avenue, Madison, WI 53706, USA. e-mail: eaazen{at}facstaff.wisc.edu

The Soa bitter-sensitivity and Prp salivary-protein loci map to distal mouse chromosome six. No recombination has been found between sucrose octaacetate (SOA)-avoidance phenotype and PRP haplotype in any mouse population. Soa and Prp, therefore, are either very near each other or identical. To assess the latter possibility, two type-A, proline-rich protein genes (MP2 and M14), situated ~30 kb apart at the Prp locus, were separately transferred from an SOA-taster inbred strain (SWR) to an SOA-nontaster inbred strain (FVB). Five MP2-transgenic mice and seven M14-transgenic mice were insensitive to 1 mM SOA in two-bottle tests, thus retaining the nontaster FVB phenotype. Each transgenic mouse was mated to control FVB mice. Their transgene-positive F1 and F2 offspring also were insensitive. Transgene expression varied among the founder lines, but SWR-like expression levels, higher than background FVB expression levels, were found in submandibular gland tissue of adult transgenic mice from two MP2 lines and one M14 line. F3 mice from one of these MP2 lines were mated to F2 mice from the M14 line. Nine offspring inherited both transgenes. All nine were insensitive to 1 mM SOA. These findings indicated that expression of mRNAs for both type-A Prp genes alone or together did not enhance SOA taste sensitivity in nontaster mice.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Chem SensesHome page
S. J. St. John and J. D. Boughter Jr
The Contribution of Taste Bud Populations to Bitter Avoidance in Mouse Strains Differentially Sensitive to Sucrose Octa-acetate and Quinine
Chem Senses, November 1, 2004; 29(9): 775 - 795.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
M. INOUE, X. LI, S. A. MCCAUGHEY, G. K. BEAUCHAMP, and A. A. BACHMANOV
Soa genotype selectively affects mouse gustatory neural responses to sucrose octaacetate
Physiol Genomics, April 27, 2001; 5(4): 181 - 186.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.