Chem. Senses 25: 67-76,
2000
© Oxford University Press 2000
Chemosensitive Conductance and Inositol 1,4,5-Trisphosphate-induced Conductance in Snake Vomeronasal Receptor Neurons
Departments of Anatomy and Cell Biology and 1 Biochemistry, State University of New York Health Science Center at Brooklyn, 450 Clarkson Avenue, NY 11203, USA
Correspondence to be sent to: Dr Mimi Halpern, Department of Anatomy and Cell Biology, SUNY Health Science Center at Brooklyn, 450 Clarkson Avenue, Box 5, Brooklyn, NY 11203, USA. e-mail: mhalpern{at}netmail.hscbklyn.edu
Snake vomeronasal receptor neurons in slice preparations were studied using the patch-clamp technique in the conventional and nystatin-perforated whole-cell configurations. The mean resting potential was approximately –70 mV; the average input resistance was 3 G
. Neurons required current injection of only 1–10 pA to display a variety of spiking patterns. Intracellular dialysis of 100 µM inositol 1,4,5-trisphosphate (IP3) evoked an inward current in 38% of neurons, with an average peak amplitude of 16.4 ± 2.8 pA at a holding potential of –70mV. Application of 100 µM 3-deoxy-3-fluoro-D-myo-inositol 1,4,5-trisphosphate (F-IP3), a derivative of IP3, also evoked an inward current in 4/8 (50%) neurons (32.6 ± 58 pA at –70 mV, n = 4). The reversal potentials of the induced components were estimated to be –14 ± 5 mV for IP3 and –17 ± 3 mV for F-IP3. Bathing the neurons in 10 µM ruthenium red solution greatly reduced the IP3-evoked inward current to 1.6 ± 1.1 pA at –70 mV (n = 6). With Cs+-containing internal solution, neither the Ca2+-ATPase inhibitor thapsigargin (1–50 µM) nor the Ca2+-ionophore ionomycin (10 µM) evoked a significant current response, suggesting that IP3 can elicit current response in the neurons without mediation by intracellular Ca2+ stores. Intracellular application of 1 mM cAMP evoked no detectable current response. Extracellular application of chemoattractant for snakes evoked a very large inward current. The reversal potential of the chemoattractant-induced current was similar to that of the IP3-induced current. The present results suggest that IP3 may act as a second messenger in the transduction of chemoattractants in the garter snake vomeronasal organ.
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