Chem. Senses 25: 173-180,
2000
© Oxford University Press 2000
Suppression of S-methylglutathione-induced Tentacle Ball Formation by Peptides and Nullification of the Suppression by TGF-ß in Hydra
Laboratory of Nutrition Chemistry, Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502 and 1 Laboratory of Physics, Kyoto Prefectural University of Medicine, Kyoto 603-8334, Japan
Correspondence to be sent to: Kazumitsu Hanai, Biosensing Laboratory, Department of Physics, Kyoto Prefectural University of Medicine, Nishitakatsukasa-cho 13, Taishogun, Kita-ku, Kyoto 603-8334, Japan. e-mail: hanai{at}koto.kpu-m.ac.jp
Tentacle ball formation (TBF) in Hydra elicited by S-methylglutathione (GSM) was modulated by a number of biologically active peptides. Hydra fed on Artemia, which had been hatched in a common salt solution supplemented with LiCl and ZnCl2, easily induced TBF in response to GSM after pretreatment with trypsin. After Hydra were treated with 100 pg/ml trypsin for 10 min, the response to GSM (TBF) was sensitively suppressed by acidic fibroblast growth factor and other biologically active peptides for >10 h. Various peptides, but not transforming growth factor beta (TGF-ß), suppressed GSM-induced TBF in a specific pattern for each peptide. However, TGF-ß was unique in that it did not suppress the response to GSM, but nullified the suppressive effect of other peptides. Only active TGF-ß nullified the suppressive effect of the peptides, and the latent form of TGF-ß neither suppressed GSM-induced TBF nor nullified the suppressive effect of other peptides. Members of the TGF-ß family suppressed GSM-induced TBF. These results indicate that all peptides examined, except for TGF-ß suppressed the response to GSM in a manner specific to each peptide. This assay system would be useful in identification of biologically active peptides.
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