Chem. Senses 25: 525-531,
2000
© Oxford University Press 2000
Comprehensive Study on G protein 
-Subunits in Taste Bud Cells, with Special Reference to the Occurrence of Gi2 as a Major G Species
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, 2 Bio-oriented Technology Research Advancement Institution, 3 Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033 and 4 Department of Nutritional Science, Faculty of Applied Biological Science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan 1 Present address: National Food Research Institute, 2-1-2 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan
Correspondence to be sent to: Keiko Abe, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. e-mail aka7308{at}mail.ecc.u-tokyo.ac.jp
Previous studies have identified many cDNA species that encode a variety of G protein 
-subunits occurring in taste buds. These include the cDNA encoding a taste-bud-specific G, gustducin (Ggust). Here we carried out comprehensive analyses of G species that occur in the taste buds of rat circumvallate papillae and also in their single cells isolated from the taste buds. Reverse transcriptase–polymerase chain reaction showed the presence of 10 kinds of G cDNAs, including a splice variant of Gs, among which Ggust, Gs, Gi2 and Gi3 cDNAs were shown to be major species. In situ hybridization and immunohistochemistry showed that Gi2, as well as Ggust, expressed in a subset of taste bud cells, and the frequency of Gi2-expression appears to be higher than that of Ggust. Southern analyses of the amplified cDNA from single cells showed that each taste bud cell expresses multiple G mRNA species. For example, some Gi2-positive cells also express one or more other G species, including Gs, Gi3 and Ggust, and there is no apparent correlation in expression among the three G species.
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