Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (30)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Nagnan-Le Meillour, P.
Right arrow Articles by Steinbrecht, R.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagnan-Le Meillour, P.
Right arrow Articles by Steinbrecht, R.A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Chem. Senses 25: 541-553, 2000
© Oxford University Press 2000

Chemosensory Proteins from the Proboscis of Mamestra brassicae

P. Nagnan-Le Meillour, A.H. Cain, E. Jacquin-Joly, M.C. François, S. Ramachandran1, R. Maida1 and R.A. Steinbrecht1

INRA, Unité de Phytopharmacie et des Médiateurs Chimiques, Bâtiment A, Route de Saint-Cyr, F-78026 Versailles Cedex, France and 1 Max-Planck-Institut für Verhaltensphysiologie, D-82319 Seewiesen, Germany

Correspondence to be sent to: P. Nagnan-Le Meillour, INRA, UPMC, Bâtiment A, Route de Saint-Cyr, F-78026 Versailles Cedex, France. e-mail: nagnan{at}versailles.inra.fr

Soluble, low molecular weight proteins were immunodetected in proboscis extracts of Mamestra brassicae males by Western blot, using antibodies raised against the general odorant-binding protein of the moth Antheraea polyphemus. The same antibodies weakly labelled the sensillum lymph and subcuticular space of sensilla styloconica on ultrathin sections of the proboscis. The morphology of sensilla styloconica is described. The immunodetected proteins yielded several N-terminal sequences, three of which showed strong affinity for tritiated analogues of pheromonal compounds of M. brassicae in binding assays. The cDNAs coding for these sequences were cloned and it was shown that the new proteins are related to the OS-D protein of Drosophila. They are named chemosensory proteins (CSP-MbraA1–CSP-MbraA5 and CSP-MbraB1 and CSP-MbraB2) and may have an odorant-binding protein-like function. A common localization in both olfaction and taste organs suggests a physiological role depending on the cellular environment.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Chem SensesHome page
J.-J. Zhou, Y. Kan, J. Antoniw, J. A. Pickett, and L. M. Field
Genome and EST Analyses and Expression of a Gene Family with Putative Functions in Insect Chemoreception
Chem Senses, June 1, 2006; 31(5): 453 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
M. Maibeche-Coisne, C. Merlin, M.-C. Francois, I. Queguiner, P. Porcheron, and E. Jacquin-Joly
Putative Odorant-degrading Esterase cDNA from the Moth Mamestra brassicae: Cloning and Expression Patterns in Male and Female Antennae
Chem Senses, June 1, 2004; 29(5): 381 - 390.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Campanacci, A. Lartigue, B. M. Hallberg, T. A. Jones, M.-T. Giudici-Orticoni, M. Tegoni, and C. Cambillau
Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding
PNAS, April 29, 2003; 100(9): 5069 - 5074.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lartigue, V. Campanacci, A. Roussel, A. M. Larsson, T. A. Jones, M. Tegoni, and C. Cambillau
X-ray Structure and Ligand Binding Study of a Moth Chemosensory Protein
J. Biol. Chem., August 23, 2002; 277(35): 32094 - 32098.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
E. Jacquin-Joly, R. G. Vogt, M.-C. Francois, and P. Nagnan-Le Meillour
Functional and Expression Pattern Analysis of Chemosensory Proteins Expressed in Antennae and Pheromonal Gland of Mamestra brassicae
Chem Senses, September 1, 2001; 26(7): 833 - 844.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.