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Chem. Senses 26: 161-166, 2001
© Oxford University Press 2001

Immunocytochemical Study of Gi2{alpha} and Go{alpha} on the Epithelium Surface of the Rat Vomeronasal Organ

Masato Matsuoka, Junko Yoshida-Matsuoka1,2, Noriko Iwasaki1, Masao Norita, Richard M. Costanzo3 and Masumi Ichikawa1,2

Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata, Niigata 951-8510, 1 Department of Developmental Morphology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, 2 CREST of the Japan Science and Technology Corporation, Kawaguchi, Saitama 332-0012, Japan and 3 Department of Physiology, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA 23298-0551, USA

Correspondence to be sent to: M. Matsuoka, Department of Neurobiology and Anatomy, Niigata University School of Medicine, Niigata, Niigata 951-8510, Japan

To investigate in detail the distribution of G protein subtypes Gi2{alpha} and Go{alpha} along the surface of the vomeronasal epithelium, we used double labeling immunocytochemical methods and electron microscopy. We examined the immunoreactivity of these surface structures with antibodies against Gi2{alpha} and Go{alpha}. Gi2{alpha}- and Go{alpha}-positive cells were observed at the epithelial surface and were evenly distributed. Electron microscopy revealed that strong immunoreactivities to both antibodies were observed on the microvilli and knob-like surface structures of receptor cells. No immunoreactivity was found on the microvilli or surface membranes of supporting cells. This expression pattern is similar to that reported for putative pheromone receptors. These data confirm that there are two distinct classes of vomeronasal receptor cells expressed at the surface of the epithelium. These two classes of receptors correspond to the same Gi2{alpha}- and Go{alpha}-positive cells distributed in cell body layers of the epithelium and in the axon terminals in the accessory olfactory bulb.


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