Chem. Senses 26: 259-265,
2001
© Oxford University Press 2001
IP3 receptor type 3 and PLCß2 are co-expressed with taste receptors T1R and T2R in rat taste bud cells
1 Bio-oriented Technology Research Advancement Institution, 1-40-2 Nisshin-cho, Oomiya, Saitama 331-0044, 2 Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657 and 3 Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Correspondence to be sent to: Misaki Asano-Miyoshi, c/o Dr Emori, Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. e-mail: amiyoshi{at}mail.ecc.u-tokyo.ac.jp
The Ca2+ signaling cascade has been reported to be activated by many tastants in vertebrate taste systems. Recently we have shown that Gi2 and phospholipase Cß2 (PLCß2) are co-expressed in a subset of taste bud cells and are possibly involved in Ca2+ triggering of taste signaling in rats. We report here that, as a component downstream of PLCß2, the type 3 isoform of the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R3) is specifically expressed in the same cells as PLCß2 in rat taste buds. We also show that cells expressing rT2R9, a probable cycloheximide receptor, are included among PLCß2- and IP3R3-positive cells, as in the case of rT1R2, a different type of taste receptor. Our findings indicate that PLCß2 and IP3R3 co-localize together with Gi2 as downstream components of two different types of taste receptors, T1R and T2R, in taste bud cells.
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