Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Scheidweiler, U.
Right arrow Articles by Schild, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scheidweiler, U.
Right arrow Articles by Schild, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Chem. Senses 26: 399-407, 2001
© Oxford University Press 2001

Slice Culture of the Olfactory Bulb of Xenopus laevis Tadpoles

Uwe Scheidweiler, Leonid Nezlin, Jörg Rabba, Birgitt Müller and Detlev Schild

Physiologisches Institut, Universität Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany

Correspondence to be sent to: Detlev Schild, Physiologisches Institut, Universität Göttingen, Humboldtallee 23, D-37073 Göttingen, Germany. e-mail: dschild{at}gwdg.de

We report on the development of a slice culture of amphibian brain tissue. In particular, we cultured slices from Xenopus laevis tadpoles that contain the olfactory mucosae, the olfactory nerves, the olfactory bulb and the telencephalon. During 6 days in roller tubes the slices flattened, starting from 250 µm and decreasing to ~40 µm, corresponding to about three cell layers. Dendritic processes could be followed over distances as long as 200 µm. Neurons in the cultured slice could be recorded using the patch clamp technique and simultaneously imaged using an inverted laser scanning microscope. We characterized the main neuron types of the olfactory bulb, i.e. mitral cells and granule cells, by correlating their typical morphological features in the acute slice with the electrophysiological properties in both the acute slice and slice culture. This correlation allowed unambiguous identification of mitral cells and granule cells in the slice culture.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T.-W. Chen, B.-J. Lin, and D. Schild
Odor coding by modules of coherent mitral/tufted cells in the vertebrate olfactory bulb
PNAS, February 17, 2009; 106(7): 2401 - 2406.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.