Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gilbertson, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gilbertson, T. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Chem. Senses 27: 383-394, 2002
© Oxford University Press 2002

Hypoosmotic Stimuli Activate a Chloride Conductance in Rat Taste Cells

Timothy A. Gilbertson

Department of Biology, Utah State University, Logan, UT 84322, USA

Correspondence to be sent to: Timothy A. Gilbertson, Department of Biology, 5305 Old Main Hill, Logan, UT 84322-5305, USA. e-mail: tag{at}biology.usu.edu

The oral cavity is subjected to a wide range of osmotic conditions, yet little is known about how solution osmolarity affects performance of the gustatory system. In order to elucidate the mechanism by which hypoosmotic stimuli affect the peripheral taste system, I have attempted to characterize the effects of hypoosmotic stimuli on individual rat taste receptor cells (TRCs) using whole-cell patch clamp recording. Currents elicited in response to voltage ramps (-90 to +60 mV) were recorded in control saline and in solutions varying only in osmolarity (-30, -60 and -90 mOsm). In roughly two-thirds of cells, hypoosmotic solutions (230 mOsm) caused a 15% increase in cell capacitance and activated a reversible conductance that exhibited marked adaptation in the continued presence of the stimulus. Similar responses could be elicited in taste cells from taste buds in the foliate and vallate papillae, the soft palate, the nasopharynx and the epiglottis. Ion substitution experiments were consistent with the interpretation that the predominant ion carried through these apparent volume- or stretch-activated channels was Cl- under normal conditions. Reversal potentials for the hypoosmotic-induced current closely matched those predicted by the Goldman—Hodgkin—Katz constant field equation for a Cl- conductance. The relative permeability sequence of the hypoosmotic-activated current in TRCs was thiocyanate- >= l- >= Br- > Cl- >= F- >= isethionate- > gluconate-. Pharmacological experiments revealed that this Cl- conductance was inhibited by 4,4'-diisothiocyanatostilbene-2, 2'-disulfonic acid and 5-nitro-3-(3-phenyl-propylamino)benzoic acid (EC50 = 1.3 and 4.6 µM, respectively), but not by CdCl2 (300 µM) nor GdCl3 (200 µM). I hypothesize that this hypoosmotic-activated Cl- conductance, which is similar to the well-characterized swelling-activated Cl- current, may contribute to volume regulation and could represent the transduction mechanism by which the presence of hypoosmotic stimuli, including water, may be signaled in taste receptor cells.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Chem SensesHome page
K. J. Watson, I. Kim, A. F. Baquero, C. A. Burks, L. Liu, and T. A. Gilbertson
Expression of Aquaporin Water Channels in Rat Taste Buds
Chem Senses, June 1, 2007; 32(5): 411 - 421.
[Abstract] [Full Text] [PDF]

Home page
 Alcohol AlcoholHome page
M. WRONSKI, D. SKROK-WOLSKA, J. SAMOCHOWIEC, M. ZIOLKOWSKI, L. SWIECICKI, P. BIENKOWSKI, A. KORKOSZ, P. ZATORSKI, W. KUKWA, and A. SCINSKA
PERCEIVED INTENSITY AND PLEASANTNESS OF SUCROSE TASTE IN MALE ALCOHOLICS
Alcohol Alcohol., March 1, 2007; 42(2): 75 - 79.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. A. DeSimone and V. Lyall
Taste Receptors in the Gastrointestinal Tract III. Salty and sour taste: sensing of sodium and protons by the tongue
Am J Physiol Gastrointest Liver Physiol, December 1, 2006; 291(6): G1005 - G1010.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Physiol.Home page
V. Lyall, H. Pasley, T.-H. T. Phan, S. Mummalaneni, G. L. Heck, A. K. Vinnikova, and J. A. DeSimone
Intracellular pH Modulates Taste Receptor Cell Volume and the Phasic Part of the Chorda Tympani Response to Acids
J. Gen. Physiol., December 27, 2005; 127(1): 15 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Huang, J. Cao, H. Wang, L. A. Vo, and J. G. Brand
Identification and Functional Characterization of a Voltage-gated Chloride Channel and Its Novel Splice Variant in Taste Bud Cells
J. Biol. Chem., October 28, 2005; 280(43): 36150 - 36157.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
S. D. Hillyard, J. Goldstein, W. Tuttle, and K. Hoff
Transcellular and Paracellular Elements of Salt Chemosensation in Toad Skin
Chem Senses, November 1, 2004; 29(9): 755 - 762.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
W. Lin, C. A. Burks, D. R. Hansen, S. C. Kinnamon, and T. A. Gilbertson
Taste Receptor Cells Express pH-Sensitive Leak K+ Channels
J Neurophysiol, November 1, 2004; 92(5): 2909 - 2919.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.