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Chem. Senses 27: 495-504, 2002
© Oxford University Press 2002

Unisex Pheromone Detectors and Pheromone-binding Proteins in Scarab Beetles

Alexander Alexeevich Nikonov1, Guihong Peng1, Galina Tsurupa1 and Walter Soares Leal1,2

1 Laboratory of Chemical Prospecting, National Institute of Agrobiological Sciences, 1-2 Ohwashi, Tsukuba 305-8634, Japan 2 Honorary Maeda-Duffey Laboratory, Department of Entomology, University of California at Davis, Davis, CA 95616, USA

Correspondence to be sent to: Walter S. Leal, Honorary Maeda-Duffey Laboratory, Department of Entomology, University of California at Davis, Davis, CA 95616, USA. E-mail: wsleal{at}ucdavis.edu

Olfaction was studied in two species of scarab beetle, Anomala octiescostata and Anomala cuprea (Coleoptera: Scarabaeidae: Rutelinae), which are temporarily isolated and use the same sex pheromone compounds, (R)-buibuilactone and (R)-japonilure. Single sensillum recordings in A. octiescostata revealed highly sensitive olfactory receptor neurons (ORNs) (threshold <1 pg) that were tuned to the detection of the green leaf volatile compound (Z)-3-hexenyl acetate. As opposed to similar ORNs in another scarab species, Phyllopertha diversa, in A. octiescostata a diazo analogue elicited much lower neuronal responses than the natural ligand. Detectors for other floral and leaf compounds were also characterized. Extremely stereoselective ORNs tuned to sex pheromone were identified in male and female antennae. Biochemical investigations showed that, in A. octiescostata and A. cuprea, the pheromone-binding proteins (PBPs) isolated from male antennae were identical to PBPs obtained from female antennae. AoctPBP and AcupPBP had seven different amino acid residues. Binding of AoctPBP to (R)-japonilure is shown. PdivOBP1, which is also known to bind to (R)-japonilure, differed from AcupPBP in only two amino acid residues, one at the N-terminus and the other near the C-terminus. The structural features of the Bombyx mori PBP are compared with the sequences of eight known scarab odorant-binding proteins.


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 Proc. Natl. Acad. Sci. USAHome page
M. Maibeche-Coisne, A. A. Nikonov, Y. Ishida, E. Jacquin-Joly, and W. S. Leal
Pheromone anosmia in a scarab beetle induced by in vivo inhibition of a pheromone-degrading enzyme
PNAS, August 3, 2004; 101(31): 11459 - 11464.
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