Chem. Senses 27: 599-610,
2002
© Oxford University Press 2002
Voltage- and Calcium-activated Currents in Cultured Olfactory Receptor Neurons of Male Mamestra brassicae (Lepidoptera)
INRA, Unité de Phytopharmacie et des Médiateurs Chimiques, Route de Saint Cyr, 78026 Versailles Cedex, France 1 CNRS, Laboratoire de Neurobiologie Cellulaire et Moléculaire, 91198 Gif-sur-Yvette, France
Correspondence to be sent to: Philippe Lucas, Unité de Phytopharmacie et des Médiateurs Chimiques, Route de Saint Cyr, 78026 Versailles Cedex, France. e-mail: plucas{at}versailles.inra.fr
Insect olfactory receptor neurons (ORNs) grown in primary cultures were
studied using the patch-clamp technique in both conventional and amphotericin
B perforated whole-cell configurations under voltage-clamp conditions. After
10-24 days in vitro, ORNs had a mean resting potential of -62 mV and
an average input resistance of 3.2 G
. Five different voltage-dependent
ionic currents were isolated: one Na+, one Ca2+ and
three K+ currents. The Na+ current (35-300 pA) activated
between -50 and -30 mV and was sensitive to 1 µM tetrodotoxin (TTX). The
sustained Ca2+ current activated between -30 and -20 mV, reached a
maximum amplitude at 0 mV (-4.5 ± 6.0 pA) that increased when
Ba2+ was added to the bath and was blocked by 1 mM Co2+.
Total outward currents were composed of three K+ currents: a
Ca2+-activated K+ current activated between -40 and -30
mV and reached a maximum amplitude at +40 mV (605 ± 351 pA); a
delayed-rectifier K+ current activated between -30 and -10 mV, had
a mean amplitude of 111 ± 67 pA at +60 mV and was inhibited by 20 mM
tetraethylammonium (TEA); and, finally, more than half of ORNs exhibited an
A-like current strongly dependent on the holding potential and inhibited by 5
mM 4-aminopyridine (4-AP). Pheromone stimulation evoked inward current as
measured by single channel recordings.
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