Chem. Senses 28: 237-243,
2003
© Oxford University Press 2003
Gurmarin Suppression of Licking Responses to Sweetener-Quinine Mixtures in C57BL Mice
National Research Institute of Fisheries Science, 2-12-4, Fukuura, Kanazawa-ku, Yokohama 236-8648 1 Department of Chemistry, Asahi University School of Dentistry, 1851-1, Hozumi-cho, Motosu-gun, Gifu 501-0296 2 Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582 3 Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19 Toranomon Minato-ku, Tokyo 105, Japan
Correspondence to be sent to: Yuzo Ninomiya, Section of Oral Neuroscience, Graduate School of Dental Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. e-mail: nino{at}dent.kyushu-u.ac.jp
Gurmarin (Gur) is a peptide that selectively suppresses responses of the
chorda tympani nerve to sweet substances in rats and mice. In the present
study, we examined the effect of Gur on behavioral responses to sweet
substances in C57BL mice. To accomplish this, we developed a new short-term
lick test and measured numbers of licks for 10 s for sweet substances mixed
with quinine hydrochloride (QHCl) in water-deprived mice. Numbers of licks for
sucrose mixed with 1 or 3 mM QHCl increased with increasing concentration of
sucrose from 0.01 to 1.0 M. Oral infusion with 30 µg/ml Gur produced
significant decreases in responses to concentration series for sucrose mixed
with 3 mM QHCl, whereas no such effect by Gur was observed in responses to
QHCl alone or QHCl-mixed HCl, NaCl or monosodium glutamate. The Gur
suppression of QHCl-mixed sucrose responses, which otherwise lasted for
2–3 h, rapidly returned to 
80% of control levels after oral
infusion with ß-cyclodextrin. These results are comparable to neural data
previously found in chorda tympani responses, and thereby provide further
evidence for Gur as a sweet response inhibitor in C57BL mice. In the other
aspect, our newly developed short-term test can also provide a tool for
measurements of taste-guided behavioral responses to sweeteners.
Key words: ß-cyclodextrin, gurmarin inhibition, licking behavior, mice, sweet taste
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