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Chem. Senses 29: 13-23, 2004
© Oxford University Press 2004

Analysis of a Human Fungiform Papillae cDNA Library and Identification of Taste-related Genes

Olivier Rossier1,*, Jie Cao2,*, Taufiqul Huque2, Andrew I. Spielman3, Roy S. Feldman4, Juan F. Medrano5, Joseph G. Brand2,4 and Johannes le Coutre1

1 Nestlé Research Center, Vers-chez-les-Blanc, Lausanne 1000, Switzerland 2 Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104-3308, USA 3 College of Dentistry, New York University, 345 E. 24th Street, New York, NY 10010, USA 4 Philadelphia Veterans Affairs Medical Center and School of Dental Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA 5 Department of Animal Science, University of California, One Shields Ave, Davis, CA 95616-8521, USA * These authors contributed equally to this work.

Correspondence to be sent to: Johannes le Coutre, Nestlé Research Center, P.O. Box 44, CH-1000 Lausanne 26, Switzerland. e-mail: johannes.le-coutre{at}rdls.nestle.com

Various genes related to early events in human gustation have recently been discovered, yet a thorough understanding of taste transduction is hampered by gaps in our knowledge of the signaling chain. As a first step toward gaining additional insight, the expression specificity of genes in human taste tissue needs to be determined. To this end, a fungiform papillae cDNA library has been generated and analyzed. For validation of the library, taste-related gene probes were used to detect known molecules. Subsequently, DNA sequence analysis was performed to identify further candidates. Of 987 clones sequenced, clustering results in 288 contigs. Comparison of these contigs with genomic databases reveals that 207 contigs (71.9%) match known genes, 16 (5.6%) match hypothetical genes, eight (2.8%) match repetitive sequences and 57 (19.8%) have no or low similarity to annotated genes. The results indicate that despite a high level of redundancy, this human fungiform cDNA library contains specific taste markers and is valuable for investigation of both known and novel taste-related genes.

Key words: cDNA library, gene expression, human fungiform papillae, signal transduction, taste marker, tastomics

All the tables in this paper are available on the Chemical Senses website (http://chemse.oupjournals.org/) with clickable links for the accession numbers.

Technical inquiries may be addressed to either brand{at}monell.org or johannes.le-coutre{at}rdls.nestle.com.


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