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Chem. Senses 29: 381-390, 2004
Chemical Senses Vol. 29 No. 5 © Oxford University Press 2004; all rights reserved

Putative Odorant-degrading Esterase cDNA from the Moth Mamestra brassicae: Cloning and Expression Patterns in Male and Female Antennae

Martine Maïbèche-Coisne1,2, Christine Merlin1,2, Marie-Christine François1, Isabelle Queguiner2, Patrick Porcheron2 and Emmanuelle Jacquin-Joly1

1 INRA, Unité de Phytopharmacie et Médiateurs Chimiques, Bât. A, route de Saint-Cyr, F-78026 Versailles Cedex, France and 2 Université Paris VI, EA3501, Laboratoire Physiologie Cellulaire des Invertébrés, 12 rue Cuvier, F-75005 Paris, France

Correspondence to be sent to: M. Maïbèche-Coisne, INRA Unité de Phytopharmacie et Médiateurs Chimiques, Bat. A, route de Saint-Cyr, F-78026 Versailles Cedex, France. e-mail: maibeche{at}versailles.inra.fr

An esterase cDNA was isolated from the cabbage armyworm Mamestra brassicae antennae by PCR strategy. The full-length cDNA, designated as Mbra-EST, contains a 1638 bp open reading frame encoding a predicted protein of 546 amino acids. This predicted protein presents the structural characteristics of known insect carboxyl-esterases, in particular the Ser–His–Glu catalytic triad. The expression pattern of the gene was studied by RT–PCR, Northern-blot and in situ hybridization. The ribosomal protein rpL8 gene from M. brassicae was also cloned to obtain a normalized tool for the comparative gene expression studies. Mbra-EST transcripts are specifically expressed in the antennae of males and females and in the proboscis of males. In antennae of both sexes, expression is restricted to the olfactory sensilla trichodea, suggesting a role in degradation of odorant acetate compounds, such as pheromones as well as plant volatile acetate components.

Key words: antennal esterase, Mamestra brassicae, odorant-degrading enzymes, olfaction


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