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Chemical Senses 2005 30(2):121-125; doi:10.1093/chemse/bji007
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Chemical Senses vol. 30 no. 2 © Oxford University Press 2005; all rights reserved.

Measurements of Several Metallic Elements and Matrix Metalloproteinases (MMPs) in Saliva from Patients with Taste Disorder

Mamoru Watanabe1, Masumi Asatsuma1, Akihiro Ikui2, Minoru Ikeda2, Yoshiaki Yamada1, Shuichi Nomura3 and Atsuko Igarashi1

1 Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Gakkocho Dori 2-5274, Niigata City, Niigata 951-8514, Japan, 2 Department of Otorhinolaryngology, Nihon University School of Medicine, Gakkocho Dori 2-5274, Niigata City, Niigata 951-8514, Japan and 3 Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Gakkocho Dori 2-5274, Niigata City, Niigata 951-8514, Japan

Correspondence to be sent to: Atsuko Igarashi, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan. e-mail: atsuko{at}dent.niigata-u.ac.jp

We have measured and compared several metallic elements and matrix metalloproteinases (MMPs) in saliva from patients with taste disorder and healthy subjects. Stimulated whole saliva was collected from 20 patients and 35 healthy subjects. Inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of metallic elements in saliva. Amounts of MMP-1, MMP-3, MMP-9 and IL-1{alpha}, IL-6 in saliva were measured using an enzyme-linked immunosorbent assay systems. Zinc in the serum was determined by flame atomic absorption spectrometry. Our results provide evidence that levels of zinc, manganese and the amount of MMP-3 in saliva are significantly decreased in the patients with taste disorder compared to the healthy subjects; Zn (p.p.b.): healthy subjects 79.8 ± 42.6, patients 47.22 ± 17.1, (P < 0.001), Mn (p.p.b.): healthy subjects 4.48 ± 2.46, patients 2.78 ± 1.23, (P < 0.004), MMP-3 (ng/ml), healthy subjects 0.820 ± 0.417, patients 0.594 ± 0.179 (P < 0.01). In contrast, copper is significantly increased in the patients; Cu (p.p.b.): healthy subjects 34.5 ± 13.5, patients 45.9 ± 20.8 (P < 0.049). These differences may be closely related with this disease. ICP-MS is an easy and accurate instrument for measurements of salivary metallic elements and may be useful in establishing a diagnosis of taste disorder.

Key words: copper, manganese, MMP-3, saliva, taste disorder, zinc


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