Chemical Senses vol. 30 no. 2 © Oxford University Press 2005; all rights reserved.
Glutamate-induced Cobalt Uptake Elicited by Kainate Receptors in Rat Taste Bud Cells
1 Department of Physiology and Neuroscience, Kangnung National University, Kangnumg, Republic of Korea, 2 Research Institute of Oral Science, Kangnung National University, Kangnung, Republic of Korea, 3 Department of Preventive Dentistry, College of Dentistry, Kangnung National University, Kangnung, Republic of Korea and 4 Department of Oral Anatomy, College of Dentistry, Seoul National University, Seoul, Republic of Korea
Correspondence to be sent to: Kyung-Nyun Kim, Department of Physiology and Neuroscience, College of Dentistry, Kangnung National University, 123 Chibyun-dong, Kangnung, Kangwon-do, 210-702, Republic of Korea. e-mail: knkim{at}kangnung.ac.kr
Glutamate-induced cobalt uptake reveals non-N-methyl-D-aspartate (non-NMDA) glutamate receptors (GluRs) in rat taste bud cells. However, it is not known which type of non-NMDA glutamate receptors is involved. We used a cobalt staining technique combined with pharmacological tests for kainate or 
-amino-3-hydroxy-5-methyl-isoxazole-propionic acid (AMPA) receptors and/or immunohistochemistry against subunits of GluRs to examine the presence of non-NMDA receptors in rat foliate tastebud cells. Cobalt uptake into taste cells was elicited by treating taste buds with glutamate, kainate or SYM 2081, a kainate receptor agonist. Treating taste buds with AMPA or fluorowillardiine did not stimulate significant cobalt uptake. Moreover, 6-cyano-7-nitro-quinoxaline-2, 3-dione significantly reduced cobalt staining elicited by glutamate or kainate receptor agonists, but SYM 2206, an AMPA receptor antagonist, did not. Immunohistochemistry against subunits of GluRs reveals GluR6 and KA1-like immunoreactivity. Moreover, most glutamate-induced cobalt-stained cells showed GluR6 and KA1-like immunoreactivity. These results suggest that glutamate-induced cobalt uptake in taste cells occurs mainly via kainate type GluRs.
Key words: AMPA, cobalt staining, foliate papilla, glutamate receptors, kainate, taste bud
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Raliou, A. Wiencis, A.-M. Pillias, A. Planchais, C. Eloit, Y. Boucher, D. Trotier, J.-P. Montmayeur, and A. Faurion Nonsynonymous single nucleotide polymorphisms in human tas1r1, tas1r3, and mGluR1 and individual taste sensitivity to glutamate Am. J. Clinical Nutrition, September 1, 2009; 90(3): 789S - 799S. [Abstract] [Full Text] [PDF]
|
|