Chemical Senses Advance Access originally published online on October 9, 2006
Chemical Senses 2007 32(1):41-49; doi:10.1093/chemse/bjl034
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Taste-Signaling Proteins Are Coexpressed in Solitary Intestinal Epithelial Cells
Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland
Correspondence to be sent to: Sami Damak, Nestlé Research Center, Vers-chez-les-Blanc, Lausanne, Switzerland. e-mail: sami.damak{at}rdls.nestle.com
 |
Abstract |
|---|
The taste system, made up of taste receptor cells clustered in taste buds at the surface of the tongue and the soft palate, plays a key role in the decision to ingest or reject food and thereby is essential in protecting organisms against harmful toxins and in selecting the most appropriate nutrients. To determine if a similar chemosensory system exists in the gastrointestinal tract, we used immunohistochemistry and real-time polymerase chain reaction (PCR) to investigate which taste-signaling molecules are expressed in the intestinal mucosa. The PCR data showed that T1r1, T1r2, T1r3, 
-gustducin, phospholipase Cß2 (PLCß2), and Trpm5 are expressed in the stomach, small intestine, and colon of mice and humans, with the exception of T1r2, which was not detected in the mouse and human stomach or in the mouse colon. Using transgenic mice expressing enhanced green fluorescent protein under the control of the Trpm5 promoter, we found colocalization of Trpm5 and -gustducin in tufted cells at the surface epithelium of the colon, but these cells did not express T1r3 or PLCß2. In the duodenal glands, 43%, 33%, and 38% of Trpm5-expressing cells also express PLCß2, T1r3, or -gustducin, respectively. The duodenal gland cells that coexpress PLCß2 and Trpm5 morphologically resemble enteroendocrine cells. We found a large degree of colocalization of Trpm5, -gustducin, T1r1, and T1r3 in tufted cells of the duodenal villi, but these cells rarely expressed PLCß2. The data suggest that these duodenal cells are possibly involved in sensing amino acids.
Key words: chemoreceptor cell, gustducin, gut, T1rs, transgenic mice, Trpm5
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L A Blackshaw, S M Brierley, and P A Hughes TRP channels: new targets for visceral pain Gut, January 1, 2010; 59(01): 126 - 135. [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Hallock, M. Tatangelo, J. Barrows, and T. E. Finger Residual Chemosensory Capabilities in Double P2X2/P2X3 Purinergic Receptor Null Mice: Intraoral or Postingestive Detection? Chem Senses, November 1, 2009; 34(9): 799 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Stearns, A. Balakrishnan, and A. Tavakkolizadeh Impact of Roux-en-Y gastric bypass surgery on rat intestinal glucose transport Am J Physiol Gastrointest Liver Physiol, November 1, 2009; 297(5): G950 - G957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kondoh, H. N. Mallick, and K. Torii Activation of the gut-brain axis by dietary glutamate and physiologic significance in energy homeostasis Am. J. Clinical Nutrition, September 1, 2009; 90(3): 832S - 837S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Wellendorph, L. D. Johansen, and H. Brauner-Osborne Molecular Pharmacology of Promiscuous Seven Transmembrane Receptors Sensing Organic Nutrients Mol. Pharmacol., September 1, 2009; 76(3): 453 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Mattes Oral Thresholds and Suprathreshold Intensity Ratings for Free Fatty Acids on 3 Tongue Sites in Humans: Implications for Transduction Mechanisms Chem Senses, June 1, 2009; 34(5): 415 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ishimaru and H. Matsunami Transient Receptor Potential (TRP) Channels and Taste Sensation Journal of Dental Research, March 1, 2009; 88(3): 212 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R L Young, K Sutherland, N Pezos, S M Brierley, M Horowitz, C K Rayner, and L A Blackshaw Expression of taste molecules in the upper gastrointestinal tract in humans with and without type 2 diabetes Gut, March 1, 2009; 58(3): 337 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zai, M. Kusano, H. Hosaka, Y. Shimoyama, A. Nagoshi, M. Maeda, O. Kawamura, and M. Mori Monosodium L-glutamate added to a high-energy, high-protein liquid diet promotes gastric emptying Am. J. Clinical Nutrition, January 1, 2009; 89(1): 431 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. J. Mace, N. Lister, E. Morgan, E. Shepherd, J. Affleck, P. Helliwell, J. R. Bronk, G. L. Kellett, D. Meredith, R. Boyd, et al. An energy supply network of nutrient absorption coordinated by calcium and T1R taste receptors in rat small intestine J. Physiol., January 1, 2009; 587(1): 195 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Glendinning How Do Predators Cope With Chemically Defended Foods? Biol. Bull., December 1, 2007; 213(3): 252 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. J. Mace, J. Affleck, N. Patel, and G. L. Kellett Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2 J. Physiol., July 1, 2007; 582(1): 379 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Palmer The Pharmacology and Signaling of Bitter, Sweet, and Umami Taste Sensing Mol. Interv., April 1, 2007; 7(2): 87 - 98. [Abstract] [Full Text] [PDF]
|
|