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Chemical Senses Advance Access originally published online on June 21, 2007
Chemical Senses 2007 32(7):697-710; doi:10.1093/chemse/bjm037
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© The Author 2007. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Transcription Profile Analysis Reveals That OBP-1F mRNA Is Downregulated in the Olfactory Mucosa Following Food Deprivation

K Badonnel1,2, J-B Denis3, M Caillol1,2, R Monnerie1,2, F Piumi4, M-C Potier5, R Salesse1,2 and C Baly1,2

1 INRA, UMR1197 Neurobiologie de l'Olfaction et de la Prise Alimentaire, Récepteurs et Communication Chimique, F-78350 Jouy en Josas, France 2 Univ Paris-Sud, UMR1197, Orsay, F-91405, France 3 INRA, Unité Mathématiques et Informatique Appliquées, F-78350 Jouy en Josas, France 4 UMR INRA/CEA Radiobiologie et Etude du Génome/Centre de Ressources Biologiques pour la Génomique des Animaux d'Elevage et d'Intérêt Economique, F-78350 Jouy en Josas, France 5 Laboratoire de Neurobiologie et Diversité Cellulaire, CNRS-ESPCI UMR7637, F-75231, Paris, France

Correspondence to be sent to: C. Baly, Unité NOPA/RCC, UMR1197 INRA-Paris 11, CRJ, Domaine de Vilvert, F-78352 Jouy-en-Josas, Cedex, France. e-mail: christine.baly{at}jouy.inra.fr


  Abstract

Neuroanatomical data show that olfactory mucosa (OM) is a possible place for interactions between nutrition and smell. A combination of differential display mRNA analysis together with a macroarray screening was developed to identify transcripts that are differentially expressed in rat OM following food deprivation. Using this method, backed on a stringent statistical analysis, we identified molecules that fell into several Gene Ontology terms including cellular and physiological process, signal transduction, and binding. Among the 15 most differentially expressed molecules, only one was upregulated, but 14 were downregulated in the fasted state among which was, unexpectedly, odorant-binding protein 1F (OBP-1F). Because of its potential relevance to olfactory physiology, we focused our further analysis on OBP-1F using in situ hybridization, quantitative polymerase chain reaction, and western blot analysis. OBP-1F was highlighted in the lateral nasal glands, but its expression (mRNA and protein) did not change following food deprivation. Only the minor fraction of OBP-1F mRNA expressed by the OM itself was downregulated following 48 h fasting. Altogether, our results suggest that the fine transcriptional control of OBP-1F in the OM following food deprivation could be efficient only at the local level, close to its site of secretion to participate in the perireceptor events of the olfactory signal reception.

Key words: food intake, lateral nasal glands, macroarray, neurotransmission, odorant-binding protein, q-PCR

Accepted 19 April 2007


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