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Chemical Senses Advance Access originally published online on February 2, 2009
Chemical Senses 2009 34(4):305-316; doi:10.1093/chemse/bjp002
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© The Author 2009. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

A Candidate Pheromone Receptor and Two Odorant Receptors of the Hawkmoth Manduca sexta

Harland M. Patch1, Rodrigo A. Velarde2, Kimberly K.O. Walden3 and Hugh M. Robertson3

1 Department of Entomology, Center for Chemical Ecology, The Pennsylvania State University, University Park, PA 16802, USA 2 Department of Biology, Wake Forest University, Winston-Salem, NC 27109, USA 3 Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

Correspondence to be sent to: Harland Patch, Department of Entomology, Center for Chemical Ecology, The Pennsylvania State University, University Park, PA 16802, USA. e-mail: hmpatch{at}psu.edu


  Abstract

In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridization labels a single cell associated with type-1 trichoid sensilla, which houses two neurons that have been previously determined to respond to the major components of the pheromone blend. The second receptor, MsextaOR2, was discovered using degenerate primers designed to conserved motifs of a unique group ORs that share as much as 88% identity. Comparison of RT-PCR, qRT-PCR, and in situ hybridization results with those of ORs in the Drosophila melanogaster Or83b subfamily shows a strong sequence and expression pattern similarity. The third receptor, MsextaOR3, was found by 5'-end sequencing of a normalized and subtracted cDNA library from male M. sexta antennae. RT-PCR and qRT-PCR show that this receptor is expressed only in male and female antennae. These are the first ORs, including a putative pheromone receptor, to be described from M. sexta.

Key words: insect, olfaction, pheromone, receptor

Accepted 2 January 2009


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