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Chemical Senses 9: 219-228,
© 1984

research-article

Inhibition of lingual lipases by lidocaine in taste and non-taste epidermis from steer tongues

Joseph G. Brand1,3, Joseph L. Rabinowitz2,3, Grafton D. Chase4 and Douglas L. Bayley1

1Monell Chemical Senses Center 3500 Market Street, Philadelphia, PA 19104, USA 2Veterans Administration Medical Center, University of Pennsylvania Philadelphia, PA 19104, USA 3Department of Biochemistry, School of Dental Medicine, University of Pennsylvania Philadelphia, PA 19104, USA 4Department of Chemistry, Philadelphia College of Pharmacy and Science Philadelphia, PA 19104, USA

When epidermal tissue from several regions of the steer tongue was incubated in buffer solutions, high percentages of free fatty acids were found. The presence of the verified phospholipase inhibitor, lidocaine, prevented the accumulation of these free fatty acids. Using [14C]tripalmitin and [14C]phosphatidyl choline as substrates for presumed lipases present in the tongue, we observed that lidocaine inhibited this activity. Experiments comparing homogenized and intact pieces of epidermal tissue suggested that the lipase activity is of both serous gland and lysosomal origin. Highest levels of lipase activity in non-homogenized tissues appeared in epidermis (taste containing) and dermis of the circumvallate papilla, reflecting the fact that the lingual serous glands which secrete a known lipase are located in and around this structure. When integrity of the lipid species present in mammalian lingual tissues needs to be preserved during biochemical experiments, it is suggested that lipase inhibitors such as lidocaine be present.


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