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Chemical Senses 2005 30(Supplement 1):i12-i13; doi:10.1093/chemse/bjh088
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Chemical Senses Vol. 30 No. suppl 1 © Oxford University Press 2005; all rights reserved

cDNA Microarray Screening for Taste-bud-specific Genes

Yuko Kusakabe1,2, Yoichiro Shindo3, Mi-Ryung Kim1, Hirohito Miura1,2, Yuzo Ninomiya2,4 and Akihiro Hino1,2

1 National Food Research Institute2 Bio-oriented Technology Research Advancement Institution3 Asahi Breweries Ltd4 Kyushu University

Correspondence to be sent to: Yuko Kusakabe, e-mail: ykusa@nfri.affrc.go.jp

Key words: annotation, expression profile, genetic information, in situ hybridization, screening

The first 10% of the full text of this article appears below.


   Introduction
 
Information about taste signaling molecules is still insufficient to understand the mechanisms of taste signal transductions, while novel taste receptors have been found recently. There are two strategies to discover novel taste signaling related genes; one strategy is homology based cloning and another strategy is global gene screening. Homology based cloning has been mainly used to find out the taste related genes, but we cannot expect the discovery of unexpected genes with critical roles in this strategy. Global gene screening can lead to the discovery of unexpected expressed genes. At present, new techniques for global gene screening are developed, for example, differential display-PCR, SAGE (serial analysis of gene expression), DNA array technology and so on. We . . . [Full Text of this Article]


   Results and discussion
 
Fabrication of cDNA microarray

Probe preparation

Microarray image analyses

Gene expression pattern analysis

Genetic information analysis


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