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Chemical Senses 21: 335-340,
© 1996

research-article

GUST27 and Closely Related G-protein-coupled Receptors are Localized in Taste Buds Together with Gi-protein {alpha}-Subunit

Yuko Kusakabe, Keiko Abe, Kentaro Tanemura1, Yasufumi Emori2 and Soichi Arai

Department of Applied Biological Chemisty, The University of Tokyo Tokyo, Japan 1Department of Veterinary Medicine, Division of Agriculture and Agriculture Life Sciences, The University of Tokyo Tokyo, Japan 2Department of Biophysics and Biochemistry, Faculty of Science, The University of Tokyo Tokyo, Japan

Correspondance to be sent to: Yuko Kusakabe, Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi 1–1–1, Bunkyo-ku, Tokyo 113, Japan

Gustatory, like olfactory signalling is probably mediated by seven-transmembrane receptors and coupling GTP-binding proteins (G proteins). We investigated the expression of a subset of these receptors and the Gi protein {alpha}-subunit by using their specific antibodies. Based on our previous finding that the mRNA for GUST27, one of these receptors, is expressed in rat lingual epithelia, we first prepared an antibody to the synthetic nonapeptide, H Ser-Tyr-Ser-Gln-lle-Ala-Ser-Ser-Leu OH, which corresponds to the third intracellular domain of GUST27 and also to those of a subset of related receptors whose occurrence can be predicted by PCR. Immunohistochemical studies with rat circumvallate papillae indicated that the anti-GUST27 antibody reacted with many of the taste buds examined, with strong signals appearing in particular taste cells. We then carried out a similar immunohistochemical experiment with an antibody to the Gi protein {alpha}-subunit and found that this subunit is also expressed in taste buds as demonstrated in the case of gustducin and transducin. Taken together, these results strongly suggest that GUST27 and closely related receptors, as well as Gi{alpha} proteins, are involved in intracellular taste signal transduction. Chem. Senses 21: 335–340, 1996.


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T. Misaka, Y. Kusakabe, Y. Emori, T. Gonoi, S. Arai, and K. Abe
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