Chem. Senses 24: 387-392,
1999
© Oxford University Press 1999
Induction of Salivary Gurmarin-binding Proteins in Rats fed Gymnema-containing Diets
1 Department of Oral Physiology, School of Dentistry, Asahi University, Hozumi, Gifu 501-0296 2 Department of Physiology, Faculty of Medicine, Tottori University, Yonago 683-0826, Japan
Correspondence to be sent to: Dr Hideo Katsukawa, Department of Oral Physiology, School of Dentistry, Asahi University, Hozumi, Gifu 501-0296, Japan. e-mail:kat{at}dent.ashai-u.ac.jp
Gymnema sylvestre, a tropical plant, contains gurmarin that selectively suppresses sucrose responses of the chorda tympani nerve in rats and mice. We investigated preference for taste solutions and saliva composition in rats fed a diet containing this plant (gymnema diet). Preference for 0.01 M sucrose and a mixture of 0.03 M sucrose and 0.03 mM quinine-HCl significantly decreased at 1–2 days after the start of the gymnema diet and subsequently returned closely to the control levels within about a week. There was no significant change in preference for NaCl, monosodium glutamate and quinine-HCl during feeding trials. Submandibular saliva of rats fed the gymnema diet for 4 and 14 days showed an inhibitory effect on immunoreaction between gurmarin and antigurmarin serum. Analyses using electrophoresis and affinity chromatography indicated that the saliva contains gurmarin binding proteins with molecular weights of 15, 16, 45, 60 and 66 kDa. These results suggest that reduction of preference for sucrose was probably caused by gurmarin contained in the gymnema diet and subsequent restoration of the preference may be due to suppression of the effect of gurmarin by salivary gurmarin binding proteins induced by the gymnema diet.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. H. Lemon, T. Imoto, and D. V. Smith Differential Gurmarin Suppression of Sweet Taste Responses in Rat Solitary Nucleus Neurons J Neurophysiol, August 1, 2003; 90(2): 911 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Murata, K. Nakashima, A. Yamada, N. Shigemura, K. Sasamoto, and Y. Ninomiya Gurmarin Suppression of Licking Responses to Sweetener-Quinine Mixtures in C57BL Mice Chem Senses, March 1, 2003; 28(3): 237 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Carugo, S. Lu, J. Luo, X. Gu, S. Liang, S. Strobl, and S. Pongor Structural analysis of free and enzyme-bound amaranth {alpha}-amylase inhibitor: classification within the knottin fold superfamily and analysis of its functional flexibility Protein Eng. Des. Sel., September 1, 2001; 14(9): 639 - 646. [Abstract] [Full Text] [PDF]
|
|