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Chem. Senses 26: 529-531, 2001
© Oxford University Press 2001

Detection and Removal of an Artefact Fatty Acid from the Binding Site of Recombinant Bombyx mori Pheromone-binding Protein

Neil J. Oldham, Jürgen Krieger1, Heinz Breer1 and Ales Svatos2

Max-Planck-Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, 1 Institute of Physiology, University of Stuttgart-Hohenheim, Garbenstrasse 30, D-70593 Stuttgart, Germany and 2 Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, Prague 6-Dejvice, CZ-166 10, Czech Republic

Correspondence to be sent to: Neil Oldham, Max-Planck-Institute for Chemical Ecology, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany. e-mail: oldham{at}ice.mpg.de

Recombinant Bombyx mori pheromone-binding protein (PBP), purified from an Escherichia coli expression system, has been found to contain (11Z)-octadecenoic acid (cis-vaccenic acid) as an artefact ligand. An efficient delipidation procedure is described to overcome what would appear to be a general problem with recombinant lepidopteran PBPs.


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