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Chem. Senses 26: 673-680, 2001
© Oxford University Press 2001

Identification of a Third Rat Odorant-binding Protein (OBP3)

Dietrich Löbel, Jörg Strotmann, Marion Jacob and Heinz Breer

Institute of Physiology, University of Hohenheim, Stuttgart, Germany

Correspondence to be sent to: Heinz Breer, Institute of Physiology, University Hohenheim, D-70593 Stuttgart, Germany. e-mail: breer{at}uni-hohenheim.de

From a rat olfactory epithelium cDNA library clones encoding a lipocalin were isolated with sequence identity to the previously described salivary-specific {alpha}-2u globulin and the N-terminal region of mouse odorant-binding proteins OBP-III and OBP-IV. In situ hybridization showed strong expression in nasal glands displaying a pattern equivalent to rat OBP1. Heterologously expressed protein was evaluated for its binding properties using spectroscopic approaches. The recombinant protein interacted with two fluorescent probes, 1-aminoanthracene (1-AMA) and 1,1'-bis(4-anilino-5-naphthalene)-sulfonic acid. 1-AMA binding was competed by several odorants with high affinity. The thermodynamic parameters of the protein–odorant interaction were determined using isothermal titration calorimetry. Due to its nasal expression and odorant-binding characteristics this protein was designated OBP3.


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