Chemical Senses Advance Access originally published online on June 12, 2007
Chemical Senses 2007 32(7):681-688; doi:10.1093/chemse/bjm035
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A New Method for Wide Frequency Range Dynamic Olfactory Stimulation and Characterization
Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia B3H1X5, Canada
Correspondence to be sent to: Andrew S. French, Department of Physiology and Biophysics, Dalhousie University, Halifax, Nova Scotia B3H1X5, Canada. e-mail: andrew.french{at}dal.ca
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Abstract |
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Sensory receptors often receive strongly dynamic, or time varying, inputs in their natural environments. Characterizing their dynamic properties requires control and measurement of the stimulus over a frequency range that equals or exceeds the receptor response. Techniques for dynamic stimulation of olfactory receptors have lagged behind other major sensory modalities because of difficulties in controlling and measuring the concentration of odorants at the receptor. We present a new method for delivering olfactory stimulation that gives linear, low-noise, wide frequency range control of odorant concentration. A servo-controlled moving bead of silicone elastomer occludes the tip of a Pasteur pipette that releases odorant plus tracer gas into a flow tube. Tracer gas serves as a surrogate indicator of odorant concentration and is measured by a photoionization detector. The system has well-defined time-dependent behavior (frequency response and impulse response functions) and gives predictable control of odorant over a significant volume surrounding the animal. The frequency range of the system is about 0–100 Hz. System characterization was based on random (white noise) stimulation, which allows more rapid and accurate estimation of dynamic behavior than deterministic signals such as sinusoids or step functions. Frequency response functions of Drosophila electroantennograms stimulated by fruit odors were used to demonstrate a typical application of the system.
Key words: antenna, Drosophila, frequency response, impulse response, olfaction, photoionization, sensory receptor
Accepted 16 April 2007
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