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Chemical Senses Advance Access published online on March 30, 2005
Chemical Senses, doi:10.1093/chemse/bji031
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© The Author 2005. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oupjournals.org
Accepted March 2, 2005

Article

Temporal changes in NCAM immunoreactivity during taste cell differentiation and cell lineage relationships in taste buds

Hirohito Miura 1*, Hiromi Kato 2, Yuko Kusakabe 1, Yuzo Ninomiya 3, and Akihiro Hino 1

1 National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan; Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19 Toranomon Minato-ku, Tokyo 105-0001, Japan
2 National Food Research Institute, 2-1-12 Kannondai, Tsukuba-shi, Ibaraki 305-8642, Japan
3 Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19 Toranomon Minato-ku, Tokyo 105-0001, Japan; Faculty of Dentistry, Kyushu University, 3-1-1 Maidashi Higashi-ku, Fukuoka-shi, Fukuoka 812-8582, Japan

* To whom correspondence should be addressed.
Hirohito Miura, E-mail: hmiura{at}affrc.go.jp


  Abstract

Neural cell adhesion molecule (NCAM) is a type III cell marker in the taste buds. In order to clarify the cell type of Mash1-expressing cells in taste buds, expression of NCAM was examined in Mash1-expressing taste cells of adult mice in comparison with gustducin- and T1r3-expressing cells, using a combination of NCAM immunohistochemistry and in situ hybridization. About 98% of Mash1-expressing cells were NCAM immunopositive (IP), suggesting that Mash1-expressing cells should be categorized as type III cells. Unexpectedly, small subsets of gustducin- and T1r3-expressing cells were also found to be NCAM-IP, contradicting previous immunohistochemical studies in rats, in which gustducin-IP cells were observed specifically in type II cells, which do not have NCAM immunoreactivity. Examinations of developing taste buds showed temporal changes in the ratio of NCAM-IP cells in gustducin- and T1r3-expressing cells; the ratio of NCAM-IP cells in these gene-expressing cells were ~90% at 0.5 days after birth and decreased markedly during development. In contrast, the majority of Mash1-expressing cells showed constant NCAM immunoreactivity throughout development. In addition, BrdU-labeling experiments showed that the differentiation of Mash1-expressing cells precedes those of gustducin- and T1r3-expressing cells in taste buds of adult mice. These results suggest that T1r3- and gustducin-expressing cells are NCAM-IP at the beginning of cell differentiation, and that NCAM immunoreactivity in gustducin- and T1r3-expressing cells might remain from the previous developmental stage expressing Mash1.

Keywords: cell lineage; Mash1; neural cell adhesion molecule (NCAM); taste bud; T1r3.
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