Chemical Senses Advance Access published online on August 31, 2005
Chemical Senses, doi:10.1093/chemse/bji053
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1 Department of Psychology and Center for Smell and Taste, University of Florida, Gainesville, FL 32611-2250, USA
* To whom correspondence should be addressed. Using a brief-access taste assay, we show in the present report that although phospholipase C
Accepted August 4, 2005
Article
PLC
2-Independent Behavioral Avoidance of Prototypical Bitter-Tasting Ligands
2 Department of Physiology and Biophysics, and Neuroscience Program, University of Miami School of Medicine, 1600 NW 10th Avenue, Miami, FL 33136, USA
Alan C. Spector, E-mail: spector{at}ufl.edu
Abstract 
2 knockout (PLC2 KO) mice are unresponsive to low- and midrange concentrations of quinine and denatonium, they do significantly avoid licking higher concentrations of these aversive compounds. PLC2 KO mice displayed no concentration-dependent licking of the prototypical sweetener sucrose but were similar to wild-type mice in their responses to citric acid and NaCl, notwithstanding some interesting exceptions. Although these findings confirm an essential role for PLC2 in taste responsiveness to sucrose and to low- to midrange concentrations of quinine and denatonium in mice as previously reported, they importantly suggest that higher concentrations of the latter two compounds, which are bitter to humans, can engage a PLC2-independent taste transduction pathway.
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