Chemical Senses Advance Access published online on January 4, 2006
Chemical Senses, doi:10.1093/chemse/bjj021
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1 Department of Physiology and Biophysics, University of Miami Miller School of Medicine (RMSB 4040), 1600 NW 10th Avenue, Miami, FL 33136, USA
* To whom correspondence should be addressed. Phospholipase C-type
Accepted November 30, 2005
Article
Faithful Expression of GFP from the PLC
Joung Woul Kim 1,
Craig Roberts 2,
Yutaka Maruyama 1,
Stephanie Berg 1,
Stephen Roper 3,
and
Nirupa Chaudhari 3 *
2 Promoter in a Functional Class of Taste Receptor Cells
2 Program in Neurosciences, Department of Physiology and Biophysics, University of Miami Miller School of Medicine (RMSB 4040), 1600 NW 10th Avenue, Miami, FL 33136, USA
3 Department of Physiology and Biophysics, University of Miami Miller School of Medicine (RMSB 4040), 1600 NW 10th Avenue, Miami, FL 33136, USA; Program in Neurosciences, Department of Physiology and Biophysics, University of Miami Miller School of Medicine (RMSB 4040), 1600 NW 10th Avenue, Miami, FL 33136, USA
Nirupa Chaudhari, E-mail: nchaudhari{at}miami.edu
Abstract 
2 (PLC2) is expressed in a subset of cells within mammalian taste buds. This enzyme is involved in the transduction of sweet, bitter, and umami stimuli and thus is believed to be a marker for gustatory sensory receptor cells. We have developed transgenic mice expressing green fluorescent protein (GFP) under the control of the PLC2 promoter to enable one to identify these cells and record their physiological activity in living preparations. Expression of GFP (especially in lines with more than one copy integrated) is strong enough to be detected in intact tissue preparations using epifluorescence microscopy. By immunohistochemistry, we confirmed that the overwhelming majority of cells expressing GFP are those that endogenously express PLC2. Expression of the GFP transgene in circumvallate papillae occurs at about the same time during development as endogenous PLC2 expression. When loaded with a calcium-sensitive dye in situ, GFP-positive taste cells produce typical Ca2+ responses to a taste stimulus, the bitter compound cycloheximide. These PLC2 promoter-GFP transgenic lines promise to be useful for studying taste transduction, sensory signal processing, and taste bud development.2; taste bud; taste-specific promoter; transgenic.
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