Chemical Senses Advance Access published online on June 1, 2006
Chemical Senses, doi:10.1093/chemse/bjj062
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1 Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, WI, USA
* To whom correspondence should be addressed. The importance of
Accepted May 8, 2006
Article
Taste Responses to Sweet Stimuli in
Vicktoria Danilova 1,
Sami Damak 2,
Robert F. Margolskee 3,
and
Göran Hellekant 4 *
-Gustducin Knockout and Wild-Type Mice
2 Department of Physiology and Biophysics, Mount Sinai College of Medicine, New York, NY, USA
3 Department of Physiology and Biophysics, Mount Sinai College of Medicine, New York, NY, USA; Howard Hughes Medical Institute, NY, USA
4 Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, WI, USA; Department of Physiology and Pharmacology, University of Minnesota, Medical School, 1035 University Drive, Duluth, MN 55812-3031, USA
Göran Hellekant, E-mail: ghelleka{at}d.umn.edu
Abstract 
-gustducin in sweet taste transduction is based on data obtained with sucrose and the artificial sweetener SC45647. Here we studied the role of -gustducin in sweet taste. We compared the behavioral and electrophysiological responses of -gustducin knockout (KO) and wild-type (WT) mice to 11 different sweeteners, representing carbohydrates, artificial sweeteners, and sweet amino acids. In behavioral experiments, over 48-h preference ratios were measured in two-bottle preference tests. In electrophysiological experiments, integrated responses of chorda tympani (CT) and glossopharyngeal (NG) nerves were recorded. We found that preference ratios of the KO mice were significantly lower than those of WT for acesulfame-K, dulcin, fructose, NC00174, D-phenylalanine, L-proline, D-tryptophan, saccharin, SC45647, sucrose, but not neotame. The nerve responses to all sweeteners, except neotame, were smaller in the KO mice than in the WT mice. The differences between the responses in WT and KO mice were more pronounced in the CT than in the NG. These data indicate that -gustducin participates in the transduction of the sweet taste in general.-gustducin; knockout mice; sweet taste; two-bottle preference.
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