Chemical Senses Advance Access published online on December 21, 2007
Chemical Senses, doi:10.1093/chemse/bjm083
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The Candidate Sour Taste Receptor, PKD2L1, Is Expressed by Type III Taste Cells in the Mouse
1 Rocky Mountain Taste & Smell Center 2 Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO 80045, USA 3 Department of Biological Sciences, University of Denver, Denver, CO 80208, USA 4 Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA 5 Present address: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan 6 Centre de Recherche en Rhumatologie et Immunologie, Centre Hospitalier Universitaire de Québec, Université Laval, Québec, QC, Canada
Correspondence to be sent to: Thomas E. Finger, Department of Cell and Developmental Biology, University of Colorado at Denver and Health Science Center, Mail Stop 8108, P.O. Box 6511, Aurora, CO 80045, USA. e-mail: tom.finger{at}uchsc.edu
 |
Abstract |
|---|
The transient receptor potential channel, PKD2L1, is reported to be a candidate receptor for sour taste based on molecular biological and functional studies. Here, we investigated the expression pattern of PKD2L1-immunoreactivity (IR) in taste buds of the mouse. PKD2L1-IR is present in a few elongate cells in each taste bud as reported previously. The PKD2L1-expressing cells are different from those expressing PLCβ2, a marker of Type II cells. Likewise PKD2L1-immunoreactive taste cells do not express ecto-ATPase which marks Type I cells. The PKD2L1-positive cells are immunoreactive for neural cell adhesion molecule, serotonin, PGP-9.5 (ubiquitin carboxy-terminal transferase), and chromogranin A, all of which are present in Type III taste cells. At the ultrastructural level, PKD2L1-immunoreactive cells form synapses onto afferent nerve fibers, another feature of Type III taste cells. These results are consistent with the idea that different taste cells in each taste bud perform distinct functions. We suggest that Type III cells are necessary for transduction and/or transmission of information about "sour", but have little or no role in transmission of taste information of other taste qualities.
Key words: gustation, mouse, polycystic kidney disease–like ion channel (PKD2L1), sour taste, taste bud cells
Accepted 19 November 2007
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Yoshida, A. Miyauchi, T. Yasuo, M. Jyotaki, Y. Murata, K. Yasumatsu, N. Shigemura, Y. Yanagawa, K. Obata, H. Ueno, et al. Discrimination of taste qualities among mouse fungiform taste bud cells J. Physiol., September 15, 2009; 587(18): 4425 - 4439. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ishimaru and H. Matsunami Transient Receptor Potential (TRP) Channels and Taste Sensation Journal of Dental Research, March 1, 2009; 88(3): 212 - 218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Meyerhof Sour taste cells functionally identified J. Physiol., June 15, 2008; 586(12): 2819 - 2819. [Full Text] [PDF]
|
|
|
|
|
|
Y. A. Huang, Y. Maruyama, R. Stimac, and S. D. Roper Presynaptic (Type III) cells in mouse taste buds sense sour (acid) taste J. Physiol., June 15, 2008; 586(12): 2903 - 2912. [Abstract] [Full Text] [PDF]
|
|